The main route, known locally as the Bader Bahn, is the only private completely electrified double-track line in Austria. It is 30.4 km long, from Wien Oper tram terminus, to Baden Josefsplatz. Another 5.6 km of track connects the WLB with ÖBB at Tribuswinkel, near Baden, and at Inzerdorf, where the WLB workshops and one of the two carriage sheds are situated - the other being at Leesdorf near Baden.

Apart from the section of line between Schedifkaplatz and the Oper terminus, and a small section of street running in Baden town centre, the line runs entirely on its' own segregated route. Within Wien, the line makes use of the Wien subway near the Südbahnhof, although alternate routes can be used if the subway is closed.

In 1996, two of the stops, at Griesfeld, south of Wiener Neudorf, and Baden-Melergrunde were moved to improve the catchment area and provide connections to and from bus feeder services. In the late 1990's the growing Shopping City, north of Wiener Neudorf, provided an explosive growth in traffic, and new three-section low-floor trains were ordered from Bombardier. These six trains were soon followed by a further eight, allowing extra services to be run, and the withdrawal of some of the earlier generation of trains, built by SGP in 1979-1989. The service frequency was increased to 6 trains per hour on the Oper - Wiener Neudorf section in September 2000. The older stock still in use are all named after members of staff.

Goods are an important part of the service on the line, and increasingly off the route as well. Originally timber and food into the city - and munitions and equipment to the Army base at Baden in the reverse direction, the WLB was granted a certificate in March 2001 allowing them to operate goods services throughout Austria on ÖBB tracks. The company currently has five main-line registered diesel locomotives, of which three are V100 type locos. The company also leases four class 1116 "Taurus" locos from Siemens Transportation. These locos have certificates allowing them to run into the Ruhr area of Germany. With German business partners duisport rail Gmb, and Far Freight Speditions- und Handelsgesellschaft mbH, now operate a regular international railway goods service, running three times a week between the container terminal WienCont and the container terminal in Duisburg, Germany, making the journey in eighteen hours. The train carried containers, swap-bodies and semi-trailers. Connecting freight services at Duisberg serve the Baltic States , the Netherlands, northern France, and Great Britain.

The Wiener Lokalbahn offer nostalgic trips along their line, using either 1927/8 - built motor coaches TW223, 230 or 231, or the kk Royal Saloon TW 200. The first three were built by Grazer Waggonfabrik, 223 in 1927 with the other two following the next year. They were rebuilt in 1976 with new controllers and wiring, and were repainted in 1990. They seat 42, and were latterly numbered 23 (223), 34 (230) and 31 (231) whilst in service. TW230 is currently stored out of use.

TW 200 was built for the line by Grazer in 1899, being the first bogie railcar the line owned. At first in general use on local short workings from Baden, increasing traffic meant that its' 20 seat capacity was frequently insufficient. It was rebuilt in 1908 to form the Imperial Saloon, and from 1911 served exclusively as the Royal family's preferred means of transportation to and from Baden, where carriages took the party to the Winter Residence. The interior was designed and executed by Otto Wagner. During the First World War, it was used by Emperor Karl to visit the Army High Command in Baden. Completely rebuilt in the 1970's, the Royal Saloon retains the Otto Wagner lamps and the specially-produced faceted driving windows. It is now owned by NÖ Lökalbahnmuseum, but is stored on the WLB.

Trains can be hired for sole use on the WLB, a return trip on the whole of the line in 2005 costing Euro 725 if the Royal Saloon is used, or Euro 550 if TW 223 or 231 is requested.

LB offer a small number of models of their bus and Taurus vehicles via their website.

A 5 Ös stamp, issued by Österreich Bundespost in March 1988, featured one of the 1988-built trains of the WLB. The stamp commemorates the centenary of the company's formation.